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Expression level of RecA (A) ROS formation (B), and PPO activity (D) in Pectobacterium brasiliense Pb1692 after exposure to naringenin (0.5–2 mM). dH 2 O or 0.8% DMSO served as controls. Bars show mean + SE from three independent experiments (8 replicates/treatment). <t>IVIS</t> fluorescence image of ROS in 96-well plates (C). One-way ANOVA with Tukey–Kramer HSD was used for (A,B). Student’s t test was used for (D). Different letters indicate significant differences ( p < 0.05).
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Expression level of RecA (A) ROS formation (B), and PPO activity (D) in Pectobacterium brasiliense Pb1692 after exposure to naringenin (0.5–2 mM). dH 2 O or 0.8% DMSO served as controls. Bars show mean + SE from three independent experiments (8 replicates/treatment). <t>IVIS</t> fluorescence image of ROS in 96-well plates (C). One-way ANOVA with Tukey–Kramer HSD was used for (A,B). Student’s t test was used for (D). Different letters indicate significant differences ( p < 0.05).
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Expression level of RecA (A) ROS formation (B), and PPO activity (D) in Pectobacterium brasiliense Pb1692 after exposure to naringenin (0.5–2 mM). dH 2 O or 0.8% DMSO served as controls. Bars show mean + SE from three independent experiments (8 replicates/treatment). IVIS fluorescence image of ROS in 96-well plates (C). One-way ANOVA with Tukey–Kramer HSD was used for (A,B). Student’s t test was used for (D). Different letters indicate significant differences ( p < 0.05).

Journal: Journal of Agricultural and Food Chemistry

Article Title: Multimodal Inhibition of Pectobacterium brasiliense Virulence by the Citrus Flavanone Naringenin

doi: 10.1021/acs.jafc.5c04312

Figure Lengend Snippet: Expression level of RecA (A) ROS formation (B), and PPO activity (D) in Pectobacterium brasiliense Pb1692 after exposure to naringenin (0.5–2 mM). dH 2 O or 0.8% DMSO served as controls. Bars show mean + SE from three independent experiments (8 replicates/treatment). IVIS fluorescence image of ROS in 96-well plates (C). One-way ANOVA with Tukey–Kramer HSD was used for (A,B). Student’s t test was used for (D). Different letters indicate significant differences ( p < 0.05).

Article Snippet: A DCFDA dependent ROS measurement assay kit was used, as well as IVIS imaging Lumina II imaging system software (PerkinElmer, USA), to analyze the florescence intensity of all treatments visually with wide lens E camera type IS1330N6337, Andor, iKon.

Techniques: Expressing, Activity Assay, Fluorescence

Expression level of RecA (A) ROS formation (B), and PPO activity (D) in Pectobacterium brasiliense Pb1692 after exposure to naringenin (0.5–2 mM). dH 2 O or 0.8% DMSO served as controls. Bars show mean + SE from three independent experiments (8 replicates/treatment). IVIS fluorescence image of ROS in 96-well plates (C). One-way ANOVA with Tukey–Kramer HSD was used for (A,B). Student’s t test was used for (D). Different letters indicate significant differences ( p < 0.05).

Journal: Journal of Agricultural and Food Chemistry

Article Title: Multimodal Inhibition of Pectobacterium brasiliense Virulence by the Citrus Flavanone Naringenin

doi: 10.1021/acs.jafc.5c04312

Figure Lengend Snippet: Expression level of RecA (A) ROS formation (B), and PPO activity (D) in Pectobacterium brasiliense Pb1692 after exposure to naringenin (0.5–2 mM). dH 2 O or 0.8% DMSO served as controls. Bars show mean + SE from three independent experiments (8 replicates/treatment). IVIS fluorescence image of ROS in 96-well plates (C). One-way ANOVA with Tukey–Kramer HSD was used for (A,B). Student’s t test was used for (D). Different letters indicate significant differences ( p < 0.05).

Article Snippet: The image of the same plate was taken with In Vivo Imaging System (IVIS) Lumina II imaging system software (PerkinElmer, USA), to analyze the florescence intensity of all treatments visually with wide lens E camera type IS1330N6337, Andor, iKon.

Techniques: Expressing, Activity Assay, Fluorescence